Figure 4.

Ergosterol peroxide (a) and ergosterol (b) suppressed the C/EBPβ DNA-binding activity in RAW264.7cells. Cells (1 × 10⁵ cells ml⁻¹) were treated with ergosterol peroxide or ergosterol for 6 h and LPS for 30 min. Then, the C/EBPβ DNA-binding activity in the nuclear extract (2 μg protein) was determined using a TransAM C/EBPβ Kit (Active Motif). Relative C/EBPβ binding activity is expressed as a percentage of that of the group treated with LPS alone (control). All assays were performed in triplicate and data are expressed as means±s.d. ^*P<0.01 (two-sided), significantly different from the group treated with LPS alone by Bonferroni-type multiple t-test.