Figure 3.

Effects of PAR₄-activating peptide (AYPGKF, 100 μM) or vehicle, in combination with KCl (50 mM) or capsaicin (1 μM), on calcium mobilization in cultured DRG neurons. (a) Mean values of relative fluorescence intensity (ΔF/F) evoked by KCl or capsaicin (caps) was significantly decreased in the presence (shaded columns) compared with the absence (open columns) of a PAR₄ selective agonist (AYPGKF-NH₂, 100 μM). Data are mean and horizontal lines show s.e.m.; ^*Significantly different from vehicle for P<0.05, n represents the number of neurons analyzed. (b) Representative traces of peak of relative fluorescence intensity values ΔF/F elicited by 50 mM KCl or 1 μM capsaicin in the presence (grey line) or absence (black line) of the PAR₄ agonist AYPGKF-NH₂ (100 μM).