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Effects of chronic manipulations in dietary fat on UCP expression. (a) Specificity of the Abcam UCP1 antibody (ab10983) comparing UCP1 immunoreactivity in gastrocnemius (GAST), heart (HRT), and BAT. The antibody detected a ∼34 kDa immunoreactive band only in BAT. (b) No differences were observed in levels of BAT UCP1 (lower) and skeletal muscle UCP3 (upper) protein in response to chronic changes in dietary fat content. BAT, brown adipose tissue; UCP, uncoupling protein.
