Figure 7.

Uric acid pretreatment improves rolling, adhesion and migration by selective inhibition of ONOO⁻. Leukocyte rolling (a) and adhesion (b) were evaluated by intravital microscopy in the mesentery 2 and 4 h after carrageenan challenge, respectively. (c) Neutrophil migration into the peritoneal cavity was measured 6 h after carrageenan challenge. The mice were pretreated s.c. with saline or uric acid (UA; 100 mg kg⁻¹). Fifteen minutes later these animals received an s.c. administration of saline, SIN-1 (3 mg kg⁻¹) or SNAP (3 mg kg⁻¹). After 15 min these animals were injected i.p. with carrageenan (500 μg per cavity). The control group was injected i.p. with saline. The results are expressed as mean±s.e.m. of ten animals per group. ^*P<0.05 compared to saline group; ^**P<0.05 compared with mice pretreated with saline and injected with carrageenan; ^#P<0.05 compared with mice pretreated with saline and SIN-1 and injected with carrageenan (ANOVA, followed by Bonferroni's test). ANOVA, analysis of variance; i.p., intraperitoneally; ONOO⁻, peroxynitrite; s.c., subcutaneously; SIN-1, 1,3-morphholinosydnonimine; SNAP, S-nitroso-N-acetylpenicillamine; UA, uric acid.