Figure 10.

Inhibitory effect of avarol-3′-thiosalicylate (TA) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor-kappa B (NF-κB) DNA binding activity in mouse skin. Nuclear extracts were isolated from skin topically treated with acetone (B), TPA (C) (10 nmol per site), or TA (10 μg μl⁻¹) 30 min before application of TPA. NF-κB-DNA binding activity was assayed by electrophoretic mobility shift assay. In lane c^*, a 50-fold excess of unlabelled oligonucleotide was added to the reaction mixture.