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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2007 May 4;35(3):447–455. doi: 10.1016/j.mcn.2007.04.004

Figure 7. TCaRE3 in the human TRKB promoter.

Figure 7

A. Sequence comparison between mouse TRKB P2 (−944/+2) and the human TRKB promoter region revealed varying degrees of sequence homology. Upper panel: The sequences of TCaRE2 and TCaRE3 (black ovals) are identical in the mouse and human promoters and fall within 100- and 35-bp regions of sequence identity (gray). Lower panel: The nucleotide sequence bracketing TCaRE3 is shown for both mouse and human TRKB; identity is indicated by the vertical lines. The TCaRE3 sequence is shown in bold. B. Mouse cortical neurons were transiently trans-fected with human TRKB-luciferase reporter genes containing either wild type or mutated (M4; Fig. 1B) TCaRE3. One day later, cultures were treated with either 50 mM KCl or 50 μM forskolin for 5 hours prior to harvesting for luciferase assays. Bars show mean ± sem (N=4). Similar results were obtained in two additional experiments.