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. 2007 Sep 20;5:9. doi: 10.1186/1479-0556-5-9

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Copyright © 2007 Trombone et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tissue distribution of PLGA microspheres after intramuscular administration. Mice received a single intramuscular injection of fluorescent microspheres (DNA-Hsp65/TDM). At various time points (between 1 day and 120 days) the total cells from several tissues (draining lymph node, spleen, thymus, lung, liver, kidney, testicle and single-cell suspension of bone marrow) were obtained for FACS analysis (for each analysis, 100.000 events were acquired). Each group consisted of three or five animals. The results were shown as the median of positive values for each time. ×: in this time the results were negative.