. Author manuscript; available in PMC: 2008 Sep 1.

Published in final edited form as: J Struct Biol. 2007 Apr 20;159(3):335–346. doi: 10.1016/j.jsb.2007.03.005

Table 1.

Datasets collected using our automated RCT/OTR application and their associated acquisition details.

Sample Type	CCD Camera	Type of Data Collection	Substrate Type	Electron Dose (per image)	Voltage KeV	Normal Defocus	Magnification	Tilt Pairs Collected
N-ethylmaleimide-sensitive fusion protein (NSF)	Gatan	RCT	Thin Carbon Negative Stain	20e^-/Å²	120	-2μm	50,000X (2.26Å/pixel)	88
GroEL chaperone complex	Tietz	OTR	CryoMesh™ Vitreous Ice	15e^-/Å²	120	-3μm	29,000X (2.85Å/pixel)	56
CopII (Sec13/31) Cages	Tietz	OTR	Thin Carbon Negative Stain	20e^-/Å²	120	-3μm	29,000X (2.85Å/pixel)	275
CopII (Sec13/31) Cages	Tietz	OTR	Quantafoil™ R2/4 Vitreous Ice	20e^-/Å²	200	-4μm	29,000X (2.85Å/pixel)	79