Table 2.
Heat capacity change for SF3b155-W7/U2AF65-dFW
| Interaction | Kd (nM) | ΔH° (kcal mol−1) | ΔG° a (kcal mol−1) | −TΔS° b (kcal mol−1) |
|---|---|---|---|---|
| SF3b155-W7 + U2AF65-dFW (30°C) | 3215 ± 14 | −10.8 ± 0.1 | −7.6 ± 0.01 | 3.2 ± 0.1 |
| SF3b155-W7 + U2AF65-dFW (25°C) | 1807 ± 93 | −11.6 ± 0.5 | −7.8 ± 0.03 | 3.8 ± 0.5 |
| SF3b155-W7 + U2AF65-dFW (20°C) | 1782 ± 65 | −8.4 ± 0.2 | −7.7 ± 0.02 | 0.7 ± 0.2 |
| Heat capacity change (ΔCp)c | −0.24 kcal mol−1 K−1 | |||
The stoichiometry of binding (n) was determined to be one within experimental error. For SF3b155r titrated with U2AF65-UHM. For each temperature, the mean parameters and standard deviations of 3 experiments are reported. The standard state (°) for these experiments is defined as 1 M concentrations of each protein at the indicated temperature.
a
Calculated using the equation ΔG° = −RT ln(Kd−1).
b
Calculated using the equation ΔG° = ΔH° − TΔS°.
c
Calculated from the slope of the linear best fit of ΔH° plotted versus temperature.