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. Author manuscript; available in PMC: 2008 Oct 15.
Published in final edited form as: Free Radic Biol Med. 2007 Jul 19;43(8):1099–1108. doi: 10.1016/j.freeradbiomed.2007.07.014

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Detection of S-glutathiolation of p21ras with an antibody directed against S-glutathione protein adducts. Lysates were obtained from bovine aortic endothelial cells either under control conditions or after exposure to oxidized low density lipoproteins (oxLDL). p21ras was immunoprecipitated, separated by SDS PAGE, and after transfer to PVDF membranes, immuno-blotted anti-GSH adduct antibody. OxLDL increased the extent of immunoreactive S-glutathione adduct, and the detection of the adduct was prevented if dithiothreitol (DTT) was added the cell lysate consistent with reduction of the disulfide. From reference [23].