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QnrE. faecalis protein protects E. coli DNA gyrase from inhibition by ofloxacin. Reaction mixtures contained 0.5 μg of pBR322 (lanes 1 to 9), 1 U of E. coli DNA gyrase (lanes 2 to 9), ofloxacin (lane 3 contained 0.25 μg/ml; lane 4, 0.5 μg/ml; lane 5, 1 μg/ml; lane 6, 5 μg/ml; lane 7, 10 μg/ml; and lane 9, 1 μg/ml), and a QnrE. faecalis-His6 tag (lanes 8 and 9, 0.9 μM). SC, supercoiled form; R, relaxed form.
