TABLE 1.
Oligonucleotide primers
| Target | Target size (bp) | Primer name | Nucleotide sequence (5′-3′)a | Restriction site |
|---|---|---|---|---|
| qnrE. faecalis | 731 | qnr-1F | AATTGATGGATCCTTTGAAAATC | BamHI |
| 731 | qnr-1R | TAAGAAAGAGTCGACAGAACCAG | SalI | |
| Internal fragment of qnrE. faecalis | 459 | qnr-2F | AAATCATTTGGATCCGCAGATTG | BamHI |
| 459 | qnr-2R | GTCAAGCCTGCAGAGGTGTTTTG | PstI | |
| Chloramphenicol acetyltransferase | 713 | CAT-F1 | AATTCACTGTCGACAAAAATTTAGG | SalI |
| 713 | CAT-R3 | AAAACTGCAGAGTCGGCATTATCTC | PstI | |
| qnrE. faecalis | 696 | qnrX-F | CCTACTCCATGGATGAAACCTTTG | NcoI |
| 696 | qnrX-R2 | AAAATCTCGAGTGTAATCACCAAACC | XhoI |
a
Restriction sites are underlined.