TABLE 3.
RT-PCR analysis of ompC, ompF, and acrA gene expression
| Gene | Strain | Relative expressiona |
|---|---|---|
| ompF | E. cloacae JC 194 | 1 |
| EcDC64 | 0.2032 | |
| EcΔacrA | 0.1931 | |
| ompC | E. cloacae JC 194 | 1 |
| EcDC64 | 0.00235 | |
| EcΔacrA | 0.00252 | |
| acrA | E. cloacae JC 194 | 1 |
| EcDC64 | 490 | |
| EcΔacrA | 0.002 | |
| EcΔacrA(pAP-2) | >1,000 | |
| EcDC64(pAP-3) | 300 |
a
Relative expression is calculated as 2−ΔCT, where −ΔCT is the ratio of the crossing point target value to the crossing point reference value. The target is the indicated bacterial isolate, whereas the reference is in all cases E. cloacae JC 194.