FIG. 5.

ConA treatment enhanced recruitment of phagocytes induced by K. pneumoniae in B6 mice. Groups of three mice were inoculated intragastrically with 2 × 10⁸ K. pneumoniae NK-5 cells per mouse, and the mice were sacrificed at day 5 postinfection. (B, D, F, and H) ConA (1 mg/kg of body weight) was administered intravenously 2 h before infection followed by ConA treatment at day 3 postinfection. Cryosections (4 μm) of frozen liver tissues were stained with anti-F4/80 (A to D) or anti-neutrophil (RB6-8C5) (E to H) monoclonal antibodies as described in Materials and Methods. (A and E) mock control; (B and F) ConA only, day 5; (C and G) K. pneumoniae only, day 5; (D and H) K. pneumoniae and ConA treatment, day 5. Red coloring indicates positive staining. The thin arrows indicate the liver abscess. Magnification, ×400.