TABLE 2.
PCR primers used for isolation of P. malariae and P. brasilianum dhfr-ts genes
| Primer | Sequence (5′ to 3′)a |
|---|---|
| DHFR-PF | ATGGARSAMSTYTSMGABGTWTTYGA |
| DHFR-PR | TTARGCRGCCATMTCCATDGHDATTTT |
| DHFR-41F | CAAAAYRTYGTRGTNATGGG |
| DHFR-41R | TAAAATTGRCATAAAATATGACAWGG |
| DHFR-42F | ATGGGRARARVHAGYTGGGA |
| DHFR-42R | TTTACATTCCATGCACAYAAAATAATTC |
| DHFR-43F | AAATGYTTYATYATWGGDGG |
| DHFR-43FIV | CCHCCWATRATRAARCATTT |
| Pmdhfr-F | ATGGAGGAAGTCTCAGACGTATTC |
| Pmdhfr-R | TTAGGCGGCCATATCCATAGTTAT |
| Pm5gsp1 | TTGCTGATAGTACTGCTGGTGTT |
| Pm5gsp2 | CTGATGGGGGAGGGATTCTTGGG |
| Pm3gsp1 | GGGAAGCAAATGGAACAAGAGAA |
| Pm3gsp2 | TGGTTTTCAATGGAGGCATTTCG |
| Pm3DVY | CGTCTGTGAGTGATGTGTATACT |
a
The designation of degenerate sequences at defined positions was made according to IUB nomenclature.