TABLE 2.
Calculated Z′ values as a measure of reproducibility of the assays with various strains
| Strain |
Z′a
|
Dynamic rangeb
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| OD determination
|
Luminescence assay
|
OD determination
|
Luminescence assay | |||||||||
| Growth plates
|
CV assay with pins
|
CV assay with plates
|
Blank | Mutant control | Growth plates | CV assay with pins | CV assay with plates | |||||
| Blank | Mutant control | Blank | Mutant control | Blank | Mutant control | |||||||
| PA14 | 0.68 | <0.1 | 0.67 | 0.60 | 0.57 | 0.37 | 0.89 | 0.82 | 0.164 (0.007) | 0.268 (0.016) | 0.967 (0.061) | 992,640 (36,261) |
| ZK2870 | <0.1 | 0.53 | 0.80 | 0.77 | 0.77 | 0.70 | 0.92 | 0.89 | 0.085 (0.006) | 0.503 (0.024) | 1.543 (0.063) | 2,006,927 (52,801) |
| PAO1 | 0.60 | <0.1 | 0.77 | 0.75 | 0.47 | 0.32 | 0.86 | 0.82 | 0.127 (0.005) | 0.330 (0.013) | 0.920 (0.057) | 1,715,298 (80,165) |
| PAO1 ΔfliC | 0.68 | NA | <0.1 | NA | <0.1 | NA | 0.87 | NA | 0.165 (0.007) | 0.110 (0.005) | 0.471 (0.043) | 225,330 (9,187) |
| PAK | 0.60 | <0.1 | 0.27 | 0.27 | <0.1 | <0.1 | 0.69 | 0.64 | 0.173 (0.007) | 0.138 (0.008) | 0.269 (0.024) | 436,591 (40,870) |
| PAK ΔfliC | 0.56 | NA | <0.1 | NA | <0.1 | NA | <0.1 | NA | 0.158 (0.006) | 0.085 (0.005) | 0.225 (0.023) | 26,611 (8,569) |
| Blank | NA | NA | NA | NA | NA | NA | NA | NA | 0.057 (0.004) | 0.091 (0.005) | 0.319 (0.031) | 156 (77) |
a
The mutant control used for the Z′ calculations for strains PA14, ZK2870, and PAO1 was PAO1 ΔfliC, whereas the mutant control used for the Z′ calculation for strain PAK was PAK ΔfliC. NA, calculation is not applicable.
b
The dynamic range data consist of raw values observed and demonstrate that luminescence had a range of 12,865 times over that for the blank control, whereas the dynamic range for the OD was a maximum of 5.5 times over that for the blank control. Standard deviations are indicated in parentheses.