TABLE 1.
Susceptibility of S. cerevisiae ATCC 201388 to antifungal compoundsa
| Plasmid | Drug | MIC-0 (μg/ml)
|
|
|---|---|---|---|
| SD (no induction) | SG (induction) | ||
| pYES2 | Ambruticin VS3 | >32 | >32 |
| Fludioxonil | >32 | >32 | |
| Vinclozolin | >32 | >32 | |
| pYES2-HIK1 | Ambruticin VS3 | >32 | 0.5 |
| Fludioxonil | >32 | 2 | |
| Vinclozolin | >32 | 16 | |
| pYES2-hik1-H736V | Ambruticin VS3 | >32 | >32 |
| Fludioxonil | >32 | >32 | |
| Vinclozolin | >32 | >32 | |
| pYES2-hik1-D1153E | Ambruticin VS3 | >32 | >32 |
| Fludioxonil | >32 | >32 | |
| Vinclozolin | >32 | >32 | |
The strains and plasmids are described in reference 14. MICs were determined using CLSI (formerly NCCLS) protocol M27, except that inocula were prepared using SD/−Ura or SG/−Ura medium (absence of uracil from medium selects for maintenance of plasmid) and RPMI was replaced with either SD/−Ura or SG/−Ura as the test medium. MICs were determined after 48 h of incubation. MIC-0 is defined as the concentration of antifungal that caused a 90% reduction in growth relative to the untreated control. SD medium contains yeast nitrogen base without amino acids (6.7 g/liter), glucose (20 g/liter), and 1× dropout solution (Clontech). SG medium is identical to SD, except that glucose was replaced with galactose (20 g/liter).