FIG. 1.
Multiplex detection on an oligonucleotide microarray in the initial study. (A) Oligonucleotide pattern printed on the array surface. H, position control; N, negative control; T, blaTEM; S, blaSHV; 9, blaCTX-M-9; 3, blaCTX-M-3; M, blaMOX; C, blaCMY; D, blaDHA; A, blaACC; E, E. cloacae chromosomal ampC; F, blaFOX; P, positive control. (B) Microarray hybridization using the PCR products from MAPCR for 10-plex detection. The concentration ratios of the sequence-specific primers to the UT primers in MAPCR were 1:1, 1:5, 1:10, and 1:20. (C) Specificity of the MAPCR-based microarray method.