TABLE 3.
Oligonucleotide capture probes used in this study
| Oligonucleotide probe | Sequence (5′-3′)a | GenBank accession no. (ESBL or reference) |
|---|---|---|
| tem-U | NH2-T12-CGACGAGCGTGACACCACG | AB194682 |
| ctx-m-9-U | NH2-T12-GGAATGGCGGTATTCAGCGTA | AJ416341 |
| ctx-m-3-U | NH2-T12-TTCGTCTCCCAGCTGTCGG | AJ416342 |
| mox-U | NH2-T12-CGCCTTGTCATCCAGCTGCA | D13304 |
| cmy-U | NH2-T12-GCTTTATCCCTAACGTCATCGGG | X78117 |
| dha-U | NH2-T12-TGTGATCCCCTTCCACT | Y16410 |
| acc-U | NH2-T12-TACTCAGCGAACCCACTTCA | AJ133121 |
| ampc-U | NH2-T12-AGGGAGGCGTTATCCGT | AJ278995 |
| mir-U | NH2-T12-TAGAGCCCAGCTCAAACAG | M37839 |
| act-U | NH2-T12-CAAGGTTTGTGGAGTGACAG | U58495 |
| fox-U | NH2-T12-CGGTGTGGGTCAGCGCGATC | X77455 |
| shv-U | NH2-T12-GGCTGGTTTATCGCCGATA | X98099 (SHV-1) |
| 238Gly | NH2-T12-CGGAGCTGGCcAGCGGGGTb | X98099 (SHV-1) |
| 238Ser | NH2-T12-CGGAGCTAGCcAGCGGGGTb | AF148851 (SHV-2) |
| 238Ala | NH2-T12-CGGAGCTGCCcAGCGGGGTb | AF301532 (SHV-29) |
| 240Glu | NH2-T12-CGGAGCTcGCGAGCGGGGTb | X98099 (SHV-1) |
| 240Lys | NH2-T12-CGGAGCTcGCAAGCGGGGTb | AF117747 (SHV-5) |
| 35Leu | NH2-T12-AATTAAACTAAGCGAAAGCC | X98099 (SHV-1) |
| 35Gln | NH2-T12-AATTAAACAAAGCGAAAGCC | X53817 (SHV-2a) |
| 43Arg | NH2-T12-TGTCGGGCCGCcTAGGCATb | X98099 (SHV-1) |
| 43Ser | NH2-T12-TGTCGGGCAGCcTAGGCATb | AF301532 (SHV-29) |
| 130Ser | NH2-T12-CATTACCATGAGCGcTAACAGc | X98099 (SHV-1) |
| 130Gly | NH2-T12-CATTACCATGGGCGcTAACAGc | SHV-10 (30) |
| 17Asp | NH2-T12-GACGCCCGCGACcCCACTAc | X98099 (SHV-1) |
| 179Ala | NH2-T12-GACGCCCGCGCCcCCACTAc | Y11069 (SHV-6) |
| 179Asn | NH2-T12-GACGCCCGCAACcCCACTAc | U92041 (SHV-8) |
| 179Gly | NH2-T12-GACGCCCGCGGCcCCACTAc | AB023477 (SHV-24) |
| Position control | NH2-TCACTTGCTTCCGTTGAGG-HEX | AC007661 |
| Positive control | NH2-T12-CCTCAACGGAAGCAAGTGAT | AC007661 |
| Target of positive control | TAMRA-ATCACTTGCTTCCGTTGAGG | AC007661 |
| Negative control | NH2-T12-CAAGCAGCCACGCCAGTAC | BC112171 |
a
All probe sequences were designed for this study. Underlining indicates true point mutations, and lowercase type indicates the nucleotides introduced to form the artificial mismatches. HEX, hexachloro-6-carboxyfluorescein; T12, 12 consecutive thymidines.
b
The artificial mismatch introduced into the probe sequence is a C/C mismatch.
c
The artificial mismatch introduced into the probe sequence is a C/T mismatch.