(A) Growth determination of C. neoformans strains H99, PCTR4-2/FAS1, and PCTR4-2/FAS2 on YPD agar containing BCS (A and D), CuSO4 (B and E), and CuSO4 with fatty acids (C and F) at 30°C and 37°C (serial 10-fold dilutions from left to right). (B) Growth curves of C. neoformans wild-type strain H99, PCTR4-2/FAS1, and PCTR4-2/FAS2 in YPD broth containing BCS, CuSO4, and CuSO4, with fatty acids at 30°C and 37°C.