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Apicoplasts distribute normally into the progeny of antibiotic-treated parasites but are abnormal in the following cycle. ACPl-GFP 3D7 strain transgenic parasites were treated with antibiotics at twice the IC50 at 96 h (100 nM azithromycin [AZ], 6 μM ciprofloxacin [CIP], 25 nM clindamycin [CLN], and 1 μM doxycycline [DOX]), and representative live parasites were imaged by fluorescence microscopy. GFP-labeled apicoplasts are green; Hoechst-stained nuclei are blue.
