FIG. 4.
GFP-labeled apicoplasts are detectable in progeny by flow cytometry. ACPl-GFP 3D7 strain transgenic parasites were evaluated for the presence of a GFP-labeled apicoplast during the trophozoite stage by flow cytometry. For each sample, half of the live parasites were stained with SYTO 16 nuclear stain to evaluate parasitemia, and the other half were unstained to determine the percentage of cells containing a fluorescent apicoplast. Counts falling below the gray horizontal line reflect uninfected erythrocytes. Cells containing a GFP-labeled apicoplast appear above the gray line in the left panel. Cells containing a SYTO 16-stained nucleus appear above the gray line in the right panel. Forward scatter (FSC) is plotted against fluorescence (FL).