TABLE 3.

All surviving progeny of treated parasites inherit an apicoplast^a

Treatment (first generation)	% Control parasitemia (SD) in second-generation trophozoites	% of parasites containing apicoplasts (SD) in second-generation trophozoites
None (control)	100	87.6 (11.4)
Azithromycin	105 (12.3)	83.4 (7.3)
Ciprofloxacin	98.6 (8.0)	81.4 (8.9)
Clindamycin	99.3 (8.7)	85.2 (7.9)
Doxycycline	97.2 (5.6)	83.6 (3.5)

^aACP_l-GFP 3D7 parasites were treated at approximately twice the IC₅₀ at 96 h from the late ring stage through the end of the life cycle (first generation). Progeny (second generation) were allowed to mature to the trophozoite stage in the absence of drug. Then live cells were analyzed to identify fluorescent apicoplasts, and SYTO 16-stained cells were counted to determine parasitemia, both using flow cytometry as described in the legend to Fig. 4. Data are means of at least three independent determinations.