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Recombinant Clostridium perfringens histidine-tagged proteins purified from Escherichia coli cells. (A) Coomassie-stained purified proteins; (B) reactivities of purified proteins to immune serum from chickens immune to NE. Lanes 1, alpha-toxin (45 kDa); lanes 2, GPD (40 kDa); lanes 3, FBA (35 kDa); lanes 4, tPFOR (67 kDa); lanes 5, HP (90 to 100 kDa); lanes M, molecular mass standards.
