TABLE 1.

Primers used to amplify genes encoding proteins used in immunization experiments

Gene	Direction	Sequence (5′-3′)	Amplicon size (bp)
Alpha-toxin	Forward	CCGCTCGAGTTGGGATGGAAAAATTGAT	1,100
	Reverse	CCGGAATTCTTTATATTATAAGTTGAATTT
HP	Forward	CCGCTCGAGGAATAAGAGAAAAATAGCAG	5,400
	Reverse	CCGGGTACCACGTTAAATAAATAGAACAT
GPD	Forward	CCGCTCGAGGGTAAAAGTAGCTATTAACGG	1,000
	Reverse	CCGGGTACCTTAGAAACTAAGCATTTTAAA
FBA	Forward	CCGCGGATCCATGGCATTAGTTAACGCAAA	900
	Reverse	CCGCCTCGAGAGCTCTGTTTACTGAACCGA
tPFOR	Forward	CCGCCTCGAGCACTTCATTAGAACCAGTTG	1,600
	Reverse	CCGCGGATCCTAGCTAAGTAGTCTTGGTCTCCGCGGATCCTAGCTAAGTAGTCTTGGTCT