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RNA expression of lack, tsa, leif, or Lbsti1 genes from L. (V.) braziliensis. Northern blot analysis was performed with lack (A), tsa (B), leif (C), lbsti (D), and α-tubulin (E) (12) ORFs as probes. Each lane contains 10 μg of total RNA of promastigotes of L. (L.) major (lane 1, positive control), promastigotes (lane 2) or amastigotes (lane 3) of L. (V.) braziliensis, or uninfected cultured macrophages (lane 4, negative control)/lane.
