TABLE 1.
Comparison of the predicted amino acids sequences of the genes lack, lmsti1, leif, and tsa cloned from L. (V.) braziliensis or other species of Leishmania
| Gene | Predicted amino acidsa
|
||||
|---|---|---|---|---|---|
| L. (V.) braziliensisb | L. (L.) major | L. (L.) amazonensis | L. (L.) chagasi | L. (L.) donovani | |
| lack | 43, 58, 99, 115, 148, 183, 231, 237, 254, 256, 277 | 31, 43, 58, 99, 148, 183, 231, 237, 254, 256, 277 | 43, 58, 99, 148, 183, 231, 237, 254, 256, 277 | 43, 58, 99, 183, 231, 237, 254, 256, 277, 312 | 43, 48, 99, 183, 231, 237, 254, 256, 277 |
| tsa | NA | 8, 14, 18, 32, 56, 58, 63, 64, 70, 74, 75, 78, 91, 99, 101, 111, 113, 115, 120, 122, 123, 136, 138, 144, 152, 181, 182, 188, 189, 191, 192 | NA | 5, 8, 10, 18, 33, 58, 61, 62, 63, 64, 70, 74, 75, 78, 91, 99, 101, 111, 113, 120, 123, 136, 138, 139, 144, 181, 182, 188, 189, 191, 192 | 5, 8, 10, 18, 33, 58, 61, 62, 63, 64, 70, 74, 75,78, 91, 99, 101, 111, 113, 120, 123, 136, 138, 139, 144, 181, 182, 188, 189, 191, 192 |
| leif | 363 | 2, 4, 6, 7, 125, 149, 384 | NA | NA | NA |
| sti1 | NA | 60, 68, 98, 158-220*, 250, 254, 270, 310, 314, 530 | NA | NA | 61, 69, 100, 106, 158-220*, 251, 271, 287, 310, 312, 316, 379 |
a
The positions of amino acid residue substitutions are based on the predicted amino acid sequences obtained by CLUSTAL W alignments of the lack, tsa, lmsti1, and leif genes from different species of Leishmania. *, predicted amino acids between positions 158 and 220 of sti1 (for details, see Fig. 1).
b
The L. (V.) braziliensis lack and leif genes had already been cloned from different parasite strains. NA, gene sequence not available in GenBank.