FIG. 5.
Degradation of signal in specimens held for an extended time period. To demonstrate stability of positive signals in this assay, the specimens from Fig. 4 were held overnight at 5°C and analyzed the following day. The drop-off in signal (3 to 26 MFI units; 12 MFI units on average) was consistent between samples and would not impact identification of positive specimens, provided that their relative antibody concentration was >50 ng/ml.