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Recognition of M. bovis and M. kansasii CFP-10-derived peptides by T cells from cattle with BTB. PBMCs from naturally infected cattle were stimulated with synthetic peptides (see Table 1 for sequence details). The readout system indicates the IFN-γ production measured by the ex vivo ELISPOT assay. The results are expressed as the mean SFCs/well, and the standard errors of the means for all values are <20% of the means. Horizontal lines indicate the cutoff for positivity (10 ΔSFCs/well).
