FIG. 4.

BoLA class II restriction of the recognition of M. bovis ESAT-6 peptide 3. Cross-reactive recognition of peptides 3, 3A, and 3B in animal 2 (A) was BoLA-DR restricted (B). M. bovis-specific recognition of peptide 3 in animal 1 (C) was BoLA-DQ restricted (D). Anti-BoLA-DR or anti-BoLA-DQ monoclonal antibody was added to peptide 3-stimulated PBMC cultures (B and D). The results are expressed as the mean numbers of IFN-γ-producing SFCs/well ± standard errors of the means. *, P < 0.05 compared to the results for the other groups (Student's t test).