FIG. 1.

Identification of bloodstream form T. theileri. Restriction fragment length polymorphism analysis using MspI/Eco571 (left) and MboII (right) digestion on PCR amplicons obtained by 18S rDNA amplification. Lane M contains the 100-bp DNA marker (MBI Fermentas, Lithuania), lane C shows the result of the restriction enzyme analysis derived from control DNA of T. theileri, whereas lanes 1 and 2 show the results derived from genomic DNA of isolated bloodstream form trypanosomes used in this study, in a 1/1 and 1/10 dilution, respectively.