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. 2007 Jul 13;6(9):1646–1655. doi: 10.1128/EC.00177-07

FIG. 5.

FIG. 5.

EhLimA associates with the cytoskeleton. Total cell lysates (T) prepared with the addition of Triton X-100 were subjected to ultracentrifugation resulting in a pellet fraction (P) containing the Triton X-100-insoluble cytoskeleton and a supernatant fraction (S) containing soluble proteins. A Western blot analysis of these fractions using anti-EhLimA antibodies shows that a significant portion of EhLimA is found in the Triton X-100-insoluble cytoskeleton fraction. Monoclonal antiactin antibodies reveal that the majority of actin is detected in this fraction as well.