FIG. 6.

(A) Expression of TgPP1 and TgLRR1 transcripts by tachyzoite and bradyzoite stages. Semiquantitative RT-PCR was performed with 10-fold serial dilutions of tachyzoite (Tz) and bradyzoite (Bz) cDNAs. To ensure that equal amounts of cDNA from each parasitic stage were being compared, actin primers were used as a control. (B) Expression of TgLRR1 and TgPP1 proteins in tachyzoite and bradyzoite stages. Western blotting analysis was performed using serum anti-TgLRR1 (lanes 1 and 2) raised against recombinant protein or serum anti-PP1 raised against PP1 (lanes 3 and 4). To ensure that equal amounts of proteins from each parasitic stage were being compared, monoclonal antibodies specific for actin were used as a control (lanes 5 and 6).