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. 2007 Jul 27;6(9):1606–1617. doi: 10.1128/EC.00260-07

FIG. 7.

FIG. 7.

Extracellular tachyzoites immunolabeled with various primary antibodies visualized with fluorescein isothiocyanate (green) or rhodamine (red). In panels A and B, the nuclei were stained with propidium iodine (PI) (red), and in panel C, they were stained with DAPI (blue). In panels A and B, immunofluorescence was obtained by confocal microscopy. (C and D) Immunofluorescence was examined with a fluorescence microscope (Leica) at a magnification of ×100, equipped with a charge-coupled-device camera (Leica) piloted by Metaview software. (A) Tachyzoites showing a nucleocytoplasmic localization for TgLRR1, with a strong nuclear labeling. The strong nuclear localization of TgLRR1 can be seen in the yellow merged color. (B) Tachyzoites showing a nucleocytoplasmic localization of TgPP1 with a substantial staining of the nucleus but a strong labeling of secretory organelles named rhoptries. (C) Immunofluorescence labeling of bradyzoite using anti-TgLRR1. (D) Immunofluorescence labeling of bradyzoite using anti-TgPP1.