FIG. 5.
Inhibition of flippase activity by treating TE with NEM and DEPC. Aliquots of TE treated with NEM (40 mM), DEPC (40 mM), or a combination of the reagents (40 mM each) were used to generate C12-NBD-PC-containing proteoliposomes with PPR in the range of 2.1 to 4.1 g/mol. Mock-treated TE was used as a control. The vesicles were assayed for flippase activity by using the dithionite method. Data are means and standard deviations for measurements from two independent experiments, each with duplicate samples.