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. 2007 Aug 22;6(10):1745–1757. doi: 10.1128/EC.00123-07

FIG. 5.

FIG. 5.

Immunolocalization of LdMCs in L. donovani. L. donovani promastigotes (Pro) and axenic amastigotes (AxAm) were processed for immunofluorescence and reacted with both anti-LdMC and anti-TcPPase antibodies. The reactivity with the anti-LdMC is shown in the red channel (panels A and E), and that of the anti-TcPPase antibody is shown in the green channel (panels B and F). Merging of the red and green channels (yellow) is shown in panels C and G. The corresponding phase-contrast images are shown in panels D and H. Bars in panels C and G, 10 μm. Panel K shows the reactivity of the anti-TcPPase antibody with lysates of L. donovani promastigotes (Ld) in Western blots. The molecular masses (in kilodaltons) of protein standards are shown to the left of the blot.