FIG. 3.

Hyperactivity of TAC1 is mediated by distinct GOF mutations. (A) Drug susceptibility testing of C. albicans tac1Δ/Δ mutant and TAC1 revertant strains with different TAC1-1-derived alleles carrying single point mutations or deletions. Drug susceptibility testing was carried out by plating serial dilutions of overnight cultures on YEPD agar plates containing different drugs as indicated. Plates were incubated for 48 h at 35°C in the absence or presence of FLC and cyclosporine at a concentration of 1 μg/ml. MIC assays were performed as described in Materials and Methods. (B) Expression levels of Cdr1p and Cdr2p of C. albicans tac1Δ/Δ mutant and TAC1 revertant strains with different TAC1-1-derived alleles carrying single point mutations or deletions. Experimental conditions were identical to those described in the legend to Fig. 1. WT, wild type.