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. 2007 Aug 31;6(10):1736–1744. doi: 10.1128/EC.00236-07

FIG. 7.

FIG. 7.

One-hybrid assay with Sfl1. A SalexA-SFL1 fusion construct was transformed into a LexOP-carrying strain in a manner similar to that done previously with GCN4 and NRG1 (30). The level of reporter gene expression was determined by the analysis of β-galactosidase activity and quantitated by the use of an X-Gal overlay assay as indicated.