FIG. 7.
One-hybrid assay with Sfl1. A SalexA-SFL1 fusion construct was transformed into a LexOP-carrying strain in a manner similar to that done previously with GCN4 and NRG1 (30). The level of reporter gene expression was determined by the analysis of β-galactosidase activity and quantitated by the use of an X-Gal overlay assay as indicated.