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Delay of pseudohyphal development of the sln1::GUN mutant. The wild-type strain, the sln1::GUN simple mutant, and the corresponding sln1+SLN1 reintegrant strain were simultaneously spotted on YCB solid medium. Observations were done at 3, 6, 12, and 24 h after spotting. This experiment was done in triplicate, and pictures present representative structures observed in each sample.
