TABLE 4.
Pseudohyphal differentiation of C. lusitaniae strains on various supplemented YCB media
| Genotypea | Length of pseudohyphae with growth in mediumb
|
|||||
|---|---|---|---|---|---|---|
| YCB | + Sorbitol (1 M) | + NaCl (0.5 M) | + KCl (0.5 M) | + MG (15 mM) | + H202 (1 mM) | |
| WT | 560 ± 5 | 206 ± 22 | 120 ± 14 | 280 ± 14 | 240 ± 14 | 387 ± 16 |
| sln1::GUN | 440 ± 24 | 0 ± 0 | 0 ± 0 | 107 ± 8 | 0 ± 0 | 0 ± 0 |
| nik1::GUN | 580 ± 5 | 220 ± 14 | 107 ± 22 | 273 ± 21 | 240 ± 5 | 167 ± 50 |
| chk1::GUN | 567 ± 8 | 207 ± 22 | 113 ± 16 | 266 ± 8 | 247 ± 8 | 280 ± 28 |
| chk1::GUN sln1::REP | 547 ± 22 | 0 ± 0 | 0 ± 0 | 93 ± 16 | 133 ± 41 | 0 ± 0 |
| chk1::REP nik1::GUN | 580 ± 14 | 207 ± 16 | 133 ± 8 | 253 ± 16 | 233 ± 8 | 260 ± 14 |
| sln1+SLN1 | 567 ± 8 | 193 ± 22 | 107 ± 16 | 240 ± 14 | 233 ± 8 | 353 ± 22 |
| chk1 sln1+SLN1 | 566 ± 8 | 180 ± 28 | 107 ± 16 | 260 ± 14 | 213 ± 8 | 353 ± 29 |
| chk1+CHK1 | 568 ± 14 | NDc | ND | ND | ND | 373 ± 8 |
| nik1+NIK1 | 560 ± 5 | ND | ND | ND | ND | 367 ± 22 |
a
Abbreviated genotypes are given. WT, wild-type strain 6936.
b
The length of pseudohyphae was measured from the edge of the spotted colony. The values (μm) are means ± standard deviations based on three individual replicates. “+” indicates YCB medium supplemented as indicated.
c
ND, not determined.