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. 2007 Aug 31;6(10):1925–1932. doi: 10.1128/EC.00251-07

TABLE 1.

Kinetic parameters of the recombinant P. grassii Fe hydrogenases for H2 evolution and uptake activitya

Enzyme Reaction Vmax (μmol of H2 min−1 mg−1) Km (μM) kcat (s−1) kcat/Km (M−1 s−1)
09A82 Evolution 2,033 229 1.8 × 105 7.9 × 108
Uptake 305 12.0 2.7 × 104 2.3 × 109
06B09 Evolution 117 10.5 1.3 × 104 1.2 × 109
a

Because H2 uptake activity of the 06B09 enzyme was not detected, the parameters could not be evaluated. The parameters were determined at 30°C with MV for H2 evolution and with MB for H2 uptake and at pH 8 for the 09A82 enzyme and pH 6 for the 06B09 enzyme.