Figure 2.

Cloning of the atr1D mutation. (A) Genetic analysis of F2 progeny from a three-factor cross between Col CER3 atr1D YI and Ler cer3 ATR1 yi localizes ATR1 between the visible markers. (B) A YAC clone contig spanning from the LFY gene northward over the ATR1 locus was constructed by hybridization and mapping analysis of end clones from LFY-containing YACs. Vertical lines represent end clones used for hybridization or detection of polymorphisms between Col and Ler. (C) λ clones spanning the ATR1 region between the left end of YAC CIC10A10 and the left end of YAC yUP17C2 were isolated from an atr1D genomic library. Genes within the ATR1 region were identified by sequence analysis and comparison with the GenBank database. Arrows indicate the direction of transcription. B, BamHI; S, SalI.