Table 1.
Fold changes of gallbladder inflammatory gene expression relative to Rag CM mice
| Gene NameA | Rag LC | T-cell LC | T-cell CM |
|---|---|---|---|
| Th1 genes | |||
| Casp1 | 6.3 (3.9–9.5)B | 2.3 (1.4–3.5) | 9.9 (6.7–13.8)* |
| Ccl1 | 1.3 (0.4–4.1) | 0.9 (0.4–1.9) | 32.9 (7.3–147.9)† |
| Ccl5 | 23.3 (13.8–38.9)‡ | 8.0 (4.4–14.4) | 26.4 (16.8–40.9)‡ |
| Ccr5 | 3.8 (2.5–5.7)‡ | 1.1 (0.5–2.2) | 5.2 (3.8–7.1)‡ |
| Cxcl 5 | 0.6 (0.4–0.8) | 1.0 (0.5–1.8) | 19.0 (5.5–65.7)† |
| Cxcl9 | 27.6 (8.8–86.6) | 4.4 (1.1–18.0) | 40.3 (22.3–72.8)‡ |
| Cxcl10 | 6.2 (3.5–11.1) | 1.0 (0.4–2.6) | 14.9 (7.8–28.2)‡,* |
| Cxcl11 | 29.8 (15.8–56.2)‡ | 15.4 (7.3–32.6) | 133.9 (63.9–280.7)‡ |
| Cxcr 3 | 9.6 (6.0–15.6)‡ | 2.7 (0.9–8.3) | 24.2 (8.4–69.2)‡ |
| Ifn-γ | 30.7 (15.4–57.9)‡ | 3.3 (0.8–13.5) | 125.4 (55.8–266.1)‡,* |
| Il1β | 3.1 (2.3–4.0)‡ | 1.1 (0.7–1.7) | 6.7 (4–11.3) ‡,* |
| IL8 rb | 2.7 (0.6–12.8) | 1.5 (0.4–5.1) | 43.0 (20.7–89.4)‡,*,† |
| Tnf-α | 6.7 (3.2–13.8) | 2.0 (1.1–3.7) | 31.0 (14.4–66.8) ‡,*,† |
| Th2 genes | |||
| Ccl22 | 4.3 (3.4–5.5)‡ | 0.5 (0.2–1.4) | 11.7 (4.3–31.6) |
| Ccr3 | 8.3 (5.7–12.1)‡ | 2.0 (1.1–3.7) | 7.2 (4.7–11.1)‡ |
| Il10ra | 5.2 (3.8–6.9)‡ | 2.8 (2.1–3.8) | 6.8 (4.5–10.3)‡ |
| Mixed function Genes | |||
| Ccl2 | 19.5 (12.1–31.4)‡ | 1.9 (0.6–5.4) | 16.0 (8.6–29.7)‡ |
| Ccl8 | 25.2 (16–40) | 10.2 (4.7–22.0) | 43.0 (22.1–83.7)‡ |
| Ccl9 | 7.9 (4.5–14.1)‡ | 1.3 (0.7–2.6) | 3.5 (1.8–6.9) |
| Ccr1 | 2.1 (1.5–2.8)* | 0.6 (0.4–0.8) | 2.2 (1.4–3.4) |
| Ccr2 | 11.8 (7.8–17.7)‡ | 2.5 (1.3–4.9) | 12.8 (9.2–17.9)‡,* |
| Ccr4 | 7.7 (2.0–29.0) | 15.9 (7.7–32.8) | 226.8 (85.2–603.4)‡,† |
| Cxcl 4 | 0.9 (0.7–1.0) | 0.45 (0.3–0.8) | 2.3 (1.5–3.6)* |
| Il2rg | 3.7 (2.4–5.7)‡ | 1.1 (0.6–2.1) | 5.5 (3.6–8.4)‡ |
| Il 15 | 7.9 (5.4–11.5)‡ | 3.3 (2.5–4.3) | 10.9 (4.3–27.9) |
| IL16 | 8.7 (6.7–11.1)‡ | 5.1 (3.8–6.9) | 8.7 (5.2–14.4)‡ |
| Ltb | 5.8 (4.2–8.0)‡ | 2.0 (1.2–3.2) | 5.2 (3.5–7.7)‡ |
| Tgfβ1 | 1.6 (1.3–2.0) | 0.7 (0.5–1.0) | 2.1 (1.7–2.5)* |
| Innate immune genes | |||
| Crp | 2.5 (2.3–2.8)‡,*,**,¥ | 1.5 (1.3–1.7) | 0.7 (0.5–1.1) |
| Undefined genes | |||
| Il1f6 | 2.0 (0.8–5.0) | 4.1 (2.9–5.8) | 73.4 (28.2–191.2)‡,+,† |
| Il1r2 | 4.7 (2.6–8.4) | 0.7 (0.2–1.9) | 22.1 (7.8–62.9)‡,*,† |
| IL17b | 32.4 (19.2–54.9) ‡,* | 1.3 (0.4–4.0) | 44.0 (14.6–132.3)‡ |
| T-cell expressed genes | |||
| Tnfsf5 (CD40L) | 0.52 (0.2–1.3) | 21.4 (8.3–55.0)‡,+,† | 40.8 (16.1–103.5) ‡,+,† |
Only genes demonstrating significant changes are displayed: Abcf1, Bcl6, Blr1, C3, Ccl3, Ccl4, Ccl6, Ccl7, Ccl12, Ccl17, Ccl19, Ccl20, Ccl22, Ccl24, Ccl25, Ccr6, Ccr7, Ccr8, Ccr9, Cxcl1, Cxcl12, Cxcl13, Cxcl15, Gpr2, Il1a, Il1f8, Il1r1, Il2rb, Il3, Il4, Il10, Il10ra, Il10rb, Il11, Il2rb, Il13, IL13ra1, Il5ra, Il6ra, Il6st, Il18, Il20, Itgam, Itgb2, Lta, Mif, Scye1, Spp1, Tnfrsf1a, Tnfrsf1b, Tollip, and Xcr1 did not demonstrate any significant changes between any of the groups and are not displayed.
Data represent fold changes and ranges compared to Rag mice developing cholesterol monohydrate crystals (expression of 1 for each gene). Ranges are based on plus or minus standard error of the mean prior to logarithmic conversion All groups represent male mice infected with Helicobacter spp. (n=5–6/group).
P<0.05 vs. T-cell LC mice
: P<0.05 vs. Rag mice (combined)
: P<0.05 vs. Rag CM mice
P<0.05 vs. T-cell CM mice
P<0.05 vs. Rag LC mice
P<0.05 vs. T-cell mice (combined)