Table 2.
Expression of TnI, TnT and Tm relative to a silver stained portion of the gel for each ssTnI construct expressed in adult myocytes 6 days after gene transfer. Expression was set to 1.0 for all non-infected control myocytes, and then expression in myocytes after gene transfer was normalized to the values in the non-infected control myocytes. There were no significant differences (p>0.05) in expression compared to control using a 1-way ANOVA and post-hoc Dunnett’s multiple comparison test.
| Control | TnI/Ag | n | TnT/Ag | n | Tm/Ag | n |
|---|---|---|---|---|---|---|
| 1 | 16 | 1 | 15 | 1 | 16 | |
| cTnI | 1.13±0.18 | 6 | 1.38±0.27 | 5 | 0.95±0.11 | 6 |
| ssTnI | 0.91±0.13 | 11 | 1.29±0.22 | 9 | 1.00±0.11 | 11 |
| ssTnIR125Q | 1.34±0.28 | 16 | 1.00±0.10 | 14 | 0.90±0.06 | 16 |
| ssTnIH132A | 1.20±0.11 | 7 | 1.12±0.09 | 6 | 0.97±0.05 | 7 |
| ssTnIV134E | 1.27±0.13 | 7 | 1.05±0.09 | 6 | 0.94±0.11 | 7 |
| ssTnIQAE | 1.29±0.21 | 13 | 1.18±0.11 | 12 | 1.05±0.11 | 13 |
| ssTnIQAETAT | 1.56±0.27 | 7 | 1.01±0.13 | 6 | 0.99±0.08 | 7 |
| ssTnIN141H | 1.24±0.49 | 5 | 1.11±0.23 | 5 | 0.80±0.15 | 5 |