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. 2007 Nov 7;2(11):e1137. doi: 10.1371/journal.pone.0001137

Figure 3. Effect of suppressed PGC-1α expression on OA-induced VSMC proliferation and migration.

Figure 3

VSMCs were transfected with siRNA S1 or the negative control (siRNA N). The interference effect was assessed by quantitative PCR (A) and western blot (B). Effects of decreased PGC-1α on OA-induced VSMC proliferation were determined by MTT assay (C) and cell counting (D). Effects of decreased PGC-1α on OA-induced VSMC migration were determined by wound healing (E) and transwell analysis (F). Data in VSMC proliferation and migration detections represent the means±SEM of 18 determinants from 3 independently prepared samples each with 6 measurements. Data in PGC-1α mRNA level detections are expressed as means±SEM of five different experiments normalized to β-actin levels. Data in PGC-1α protein level detections are expressed as means±SEM of four different experiments normalized to GAPDH levels. **P<0.01, #P<0.001 vs. control or N group.