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. 1998 May 12;95(10):5661–5666. doi: 10.1073/pnas.95.10.5661

Figure 1.

Figure 1

Screening for overlapping BAC clones by PCR with arbitrary primer A10. (A) AP-PCR products amplified from primary BAC DNA pools with primer A10. Only a portion of the pools from the three dimensions (plate, row, and column) are shown. The arrowhead indicates the 650-bp band (locus) analyzed in B. The kb ladder was used as molecular size markers (GIBCO/Life Technology). (B) Screening of selected secondary BAC DNA pools with primer A10. The arrowhead indicates the same 650-bp band shown in A. (C) AP-PCR on the three putative overlapping clones with primer A10. The arrowhead indicates the same 650-bp band. (D) DNA fingerprinting of the three overlapping BAC clones with 6N9 as probe. The arrowhead indicates the 3.3-kb band that was cloned and partially sequenced from both ends of cloned fragments. (E) Contig of the three overlapping clones based on DNA fingerprints in D. (F) DNA sequences from both ends of the 3.3-kb fragment of the three overlapping BAC clones.