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. 2007 Nov;171(5):1451–1461. doi: 10.2353/ajpath.2007.070136

Figure 4.

Figure 4

mRNA levels of HAS isoenzymes in response to TGF-β1 in skin fibroblasts in vitro. Human skin fibroblasts were serum-starved for 24 hours and stimulated with either 1 or 10 ng/ml TGF-β1. mRNA levels of HAS isoforms were determined by real-time PCR at 6 and 24 hours. The data are normalized to the expression levels of serum-starved control fibroblasts. HAS1 (A and B), HAS2 (C and D), and HAS3 (E and F) mRNA at the indicated times after stimulation. Data are means ± SEM of n = 4 independent experiments in skin fibroblasts derived from two different donors. P values were calculated by analysis of variance and Bonferroni’s post test to compare selected pairs; mean ± SEM; *P values <0.05; **P values <0.01.