Figure 8.

Demethylation analysis with 5-aza-dC induced an increase in Cables expression in methylated colon cancer cell lines but not in unmethylated colon cell lines. A: Trichostatin A treatment showed no increase of Cables in methylated and unmethylated colon cancer cell lines. For real-time RT-PCR, the Cables expression was normalized to that of β-actin. All experiments were repeated a minimum of three times independently. B: 5-Aza-dC-treated cell lines revealed demethylation in CpG sites confirmed by cloning sequencing. Each column represents methylation status for the 9 CpG sites between −628 and −409 bp from transcription start of Cables (A). ▪, methylated CpG sites; □, unmethylated CpG sites. Ten clones were evaluated from selected cell line.