Table 1.
Marker Profile of CAFs, Portal LFs, perisinusoidal HSCs, and Smooth Muscle Cells (SMCs) from Vessel Walls in Human Tissue Samples and of Cultured CAFs and LFs, and Antibodies Used for Immunocytochemistry
| Human tissue
|
Cultured cells
|
Antibody (dilution; clone; provider) | |||||
|---|---|---|---|---|---|---|---|
| CAFs | LFs | HSCs | SMCs | CAFs | LFs | ||
| Vimentin | + | + | + | + | + | + | 1/50; V9; Dako, Glostrup, Denmark |
| Thy-1 | + | + | − | + | + | + | 1/25; 5E10; BD Biosciences, Bedford, MA |
| α-SMA | + | + | − | + | + | + | 1/50; 1A4; Dako |
| Desmin | − | − | − | + | − | − | 1/50; D33; Dako |
| CD45 | − | − | − | − | − | − | 1/50; T29/33; Dako |
| ICAM-1 | + | ± | ± | ± | ± | ± | 1/50; 6.5B5; Dako |
| NCAM | − | − | − | − | − | − | 1/25; T199; Dako |
| Glial fibrillary acidic protein | − | − | − | − | − | − | 1/25; 6F2; Dako |
| CK-7 | − | − | − | − | ± | ± | 1/50; OV-TL 12/30; Dako |
| CK-19 | − | − | − | − | − | − | 1/100; RCK108; Dako |
| Laminin | + | + | ± | + | + | + | 1/25; 4C7; Dako |
CK, cytokeratin; NCAM, neural cell adhesion molecule.
+, strongly positive; ±, mildly positive; −, not detected.